Fascination About flow cytometers
Fascination About flow cytometers
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Details-enriched characterization of cell populations. Morphological facts from images can include to the richness of flow cytometry info. For instance, the figure displays an usually traditional apoptosis assay working with Annexin V and PI, introducing cell imaging to characterize cells in Each and every population to reveal morphologically distinctive functions. These insights could not are acquired from multiplex staining on your own.
As samples are acquired over the Attune CytPix Flow Cytometer, the significant-speed brightfield digicam captures and outlets visuals of detected functions in a charge as many as 6,000 images per second, dependant upon the flow rate and graphic measurement. For better overall flexibility, Attune Cytometric Application helps you to adjust the impression capture frequency as needed and select a certain gate for graphic capture, if required.
Mix acoustic concentrating technologies with significant-parameter Assessment for flexible spectral unmixing and standard workflows.
Learn new insights into your experimental knowledge by visualizing populations utilizing merged fluorescence and morphology data. Our application interface wizards enable downsampling, concatenation (merged information) and dimensionality applications that happen to be developed into your application for an intuitive, streamlined details Examination experience.
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Right here, an experienced consumer has gated singlets confidently. Just after evaluating the handbook singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate contains more than 4% aggregates.
Pair your Attune flow cytometer using an autosampler for increased workflow efficiency, and add robotic integration for optimum throughput.
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Early signs of this contamination experienced at first been dismissed as debris. Backgating to find new populations. Backgating imaged cells around the Attune CytPix Flow Cytometer also lets you use morphological functions to find intriguing subpopulations that might not be evident from flow cytometry knowledge on your own.
Consumers can see event visuals in genuine-time to confirm sample top quality, enhance protocols, and record the morphology of each and every cell population identified for analysis. Imaging capture also Rewards from acoustic focusing, which really helps to posture the cells making sure that a pointy, centered picture is attained.
Audio waves help specific mobile alignment, resulting in Remarkable facts good quality and ~10X more quickly sample flow prices.
We Earlier shown the strength of imaging Car or truck-T/Ramos cell interactions. Enable’s look at just the population of best fascination, the double favourable events, To find out more. We are able to now use prolonged morphological parameters (in this article, circularity and skewness of intensity) to more look at the attributes of those populations and refine gating on these situations, increasing information robustness.
Designed-in dimensionality reduction resources elevated resolution into populations. Fluorescently gated populations back-gated onto UMAP plot to substantiate anticipated population resolution in lysed total human blood. Person can watch situations from Each individual cluster for visual affirmation of mobile phenotypes.
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Gating on these prolonged parameters permits you to swiftly and correctly establish populations of curiosity and make sure gating technique with little if any manual assessment. The Attune application presents access to point out-of-the-art device Finding out Evaluation instruments in an expertly designed person interface, which makes it extremely user friendly.
Because the mobile line had previously been analyzed within the Attune CytPix Flow Cytometer, the researchers went back again to the pictures and were being in the position to document the microbial an infection at the least five days before.
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What appeared to be a homogeneous populace is in fact far more numerous—an Perception that should be regarded when interpreting final results.